Bio-rad S3™ Cell Sorter Uživatelský manuál stáhnout pdf (Strana 79)

Instruction Manual | 69

6.2 Sort Modes

Table 24 describes the three sort modes available when setting up a sort. Sort modes can be

selected for each sort direction.

Table 24. Sort mode deﬁnitions.

Sort Mode Deﬁnition When to Use

Single Cell This mode requires that one and only one positive event be in the Single cell deposition

center of the droplet without any negative events nearby. Recovery

will be reduced due to the sort restrictions

Purity This mode will sort as long as no negative events are present in the General sorts where both purity

droplet. If multiple positive events are present, sorting will still occur and recovery are important

Enrich This mode will sort all positive events regardless if negative events Sorts when recovery is critical

are nearby. Purity will be reduced to maintain high recovery and purity is not as important

6.3 Sort Statistics

The sort statistics window can be viewed during

a sort and added to a print page for reporting

(Figure 75). This window will automatically open

when a sort is started.

If a saved partial FCS ﬁle is opened, aggregate

statistics can be shown:

1. File > Open FCS File with Protocol.

2. Click on the Home tab and click Sort

Statistics.

3. Check the Show Aggregate Statistics box

to view statistics from all sorted events.

Fig. 74. Sort Logic window with sort priority and sort modes highlighted.

Fig. 75. Sort Statistics window.

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Bio-rad S3™ Cell Sorter Uživatelský manuál Strana 79