Bio-rad S3™ Cell Sorter Uživatelský manuál Strana 123
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Instruction Manual | 113
Troubleshooting
Table 30. Troubleshooting table (continued).
Error Possible Causes Troubleshooting Steps
High event rate (while trying Drop drive noise causing Toggle between the FSC/SSC trigger (in the
to run QC, or running a excessive event rate service panel)
sample at 500 eps) (>40,000 eps)
Perform a nozzle tip swap
Calibrate droplets (located in the Edit Droplets
window when logged in as administrator)
Too much light. Event rate
Light leak in system, ensure all covers are closed
(~8,000 eps)
Check that the filter blocks or filters have not been
removed or that they are seated in proper position
If triggering on FSC, may be misaligned or ND
filter removed
Obscuration bar may need adjustment. Contact
Bio-Rad Technical Support
Inappropriate sample pressure. Perform Calibrate Sample Pressure Offset
Event rate (~2,000 eps)
Running samples at above Too much light Obscuration bar may be flat and may need
2,000 eps and getting adjustment. Contact Bio-Rad Technical Support
40,000 eps
Threshold may be set too low, raise threshold value
Signal intensity is too bright. Add ND filter
Poor drop delay (uneven V, Bad side streams or center Set defanning manually until the center stream is
flat on one side) stream solid and tight
Adjust phase to ensure the side streams are tight
Recalibrate droplets located in Droplet Control
window on the admin tab or at the end of a nozzle
tip swap procedure
Side stream deflection not set properly. Adjust side
stream deflection on the admin tab
Very little volume is used Pinch in the sample line If sample line is partially clogged, run clean cycle
while performing cleaning multiple times with backflushing to remove clog
user logout or prior to
(after system shutdown)
Uses 2 ml of 10% bleach/
water takes approx. 1 min
Usually 2 ml of 70% ethanol
takes approx. 2 min
Waste Trough Filling, System Waste trough port clogged Clear debris from waste trough port and rinse with
goes into Safe Mode and overfilling bleach. Restart instrument and computer.
Trough is empty, droplet Check to see if trough has liquid or if waste trough
trapped on sensor that causes port is clogged. If not use canned air to clear
sensor to trip indiscriminately droplet form sensor as a temporary fix. Restart
instrument and computer. Contact service support
for repair or replacement.
Variable event rate during Agitator seal may be leaky Turn off the agitator to see if it resolves the varying
acquisition (changes at event rate. If it does, contact service support for
regular intervals) replacement.
Possible nozzle clog Clean nozzle tip
Hardware issue Contact Bio-Rad Technical Support
Waste container is Hydrophobic filter is wet Contact service support for replacement.
abnormally expanded
continues
If only a very small amount of liquid (200–300 µl)
is taken from the tube containing the cleaning
solution during a cleaning protocol, the sample
line may be pinched, and may need replacement.
Sometimes massaging the pinched area in the line
(if visible) may resolve the issue. Contact Bio-Rad
Technical Support
- Cell Sorters 1
- Legal Notices 3
- Bio-Rad Technical Support 3
- Safety Warning Labels 5
- Instrument Warning Labels 5
- Regulatory Compliance 6
- Biohazards 6
- Table of Contents 9
- Introduction 11
- 1.1 System Components 12
- 1.2 Installation Requirements 12
- Hardware Description 13
- 2.1.1 Instrument Back Panel 14
- 2.1.2 Aerosol Evacuation Port 14
- 2.2 Fluidics System 15
- 2.2.2 Loading Stage 16
- Instruction Manual 17
- 2.2.3 Nozzle 18
- 2.2.4 Sort Collection Chamber 18
- 2.3 Optics 19
- 2.4 Electronics 21
- Software 23
- 3.1 Main Software Window 24
- 3.1.1 Title Bar Buttons 25
- 3.1.2 File Menu 25
- 3.1.3 Home Tab Toolbar 26
- 3.2 Control Panel 30
- 3.3 Administrator Tab Toolbar 32
- 3.3.1 Editing QC Criteria 33
- 3.3.2 Edit Droplets 34
- 3.3.3 Edit Streams 34
- Instruction Manual 35
- Test Pattern highlighted 35
- 3.3.4 User Reports 36
- 3.3.5 Managing Users 37
- 3.3.6 Decontaminate 38
- 3.3.8 Global Preferences 39
- Boost Adjustment 40
- Force Cleaning 40
- Default Plot Statistics 40
- Plot Defaults 40
- Parameter Names 41
- Default Save Location 41
- Emergency Contact Information 41
- Getting Started 43
- 4.2 Logging In 44
- 4.3 Daily Startup 45
- 4.4 Quality Control 46
- 4.4.1 Droplet Setup 47
- 4.4.2 Side Streams 48
- 4.4.3 Alignment 49
- 4.4.5 Drop Delay Maintenance 50
- 4.4.6 Recalculate Drop Delay 51
- 4.4.7 QC Criteria 51
- 4.4.8 QC Report 51
- 4.5 Protocols and Workspace 52
- 4.5.3 Saving Protocols 53
- 4.5.5 Opening FCS Files 54
- 4.5.6 Saving FCS Files 55
- 4.5.10 Changing Parameters 56
- 4.5.11 Viewing Statistics 57
- 4.5.13 Creating Regions 58
- 4.5.14 Creating Gates 59
- Acquisition 61
- 5.1.1 Target Event Rate 62
- 5.1.2 Event Limit 63
- 5.1.3 Cycle Mode 63
- 5.1.4 Trigger 63
- 5.1.7 Auto Save 64
- 5.1.5 Threshold 64
- 5.1.6 PMT Voltages 64
- 5.2 Compensation 65
- Instruction Manual 67
- Fig. 61. Compensation matrix 67
- 5.2.2 Manual Compensation 69
- 5.3.1 Hot Swap 70
- Instruction Manual 71
- 5.4 Optical Filters 72
- 5.4.2 Optical Filter Blocks 73
- 6.1 Sort Setup 75
- Fig. 70. Sort Logic window 76
- 6.1.1 Sort Collection 77
- 6.1.2 Volume Tracker 77
- 6.1.3 Setting Regions to Sort 78
- 6.2 Sort Modes 79
- 6.3 Sort Statistics 79
- 6.4 Sort Plots 81
- Additional Software Features 83
- 7.4 Clean System 84
- 7.5 Instrument Status Box 85
- 7.6 Status Bar 85
- 7.8 Quality Control Reports 86
- 7.7 Printing 86
- 7.9 User Reports 87
- 7.10 Biosafety System 88
- Shutdown 89
- Instruction Manual 91
- 8.1 Daily Shutdown 91
- Automatic Startup 93
- 10.2 Dealing with Clogs 98
- Maintenance 100
- 10.4 Swap Nozzle Tip Wizard 101
- Instruction Manual 101
- Instruction Manual 103
- Instruction Manual 105
- Cleaning the nozzle tip: 108
- Sonication: 108
- Syringe wash: 108
- Instruction Manual 109
- Instruction Manual 111
- Instruction Manual 113
- 10.5 Optical Filter Cleaning 115
- 10.6 Disinfectants 115
- 10.7 Decontamination 115
- Instruction Manual 119
- Troubleshooting 121
- References 125
- and S3e 127
- Cell Sorter 127
- Specifications 127
- Ordering Information 129
- Life Science 132
- Bio-Rad 132
- Laboratories, Inc 132
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