Bio-rad S3™ Cell Sorter Uživatelský manuál Strana 129
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Hodnocené. / 5. Na základě hodnocení zákazníků
Instruction Manual | 119
Ordering Information
Catalog # Description
Instrumentation
145-1001 S3 Cell Sorter (488 nm)
145-1002 S3 Cell Sorter (488/561 nm)
145-1005 S3e Cell Sorter (488 nm)
145-1006 S3e Cell Sorter (488/561 nm)
145-1078 S3 Biosafety System Class I
145-1021 S3 Cell Sorter (488 nm), with S3 Biosafety System Class I
145-1022 S3 Cell Sorter (488/561 nm), with S3 Biosafety System Class I
145-1029 S3e Cell Sorter (488 nm), with S3 Biosafety System Class I
145-1030 S3e Cell Sorter (488/561 nm), with S3 Biosafety System Class I
Consumables
145-1081 ProLine Calibration Beads, 3 x 5 ml
145-1082 ProFlow 8x Sheath Fluid, 5 x 4 L
145-1083 ProFlow Sort Grade Water, 5 x 4 L
145-1085 ProLine Rainbow Beads, 1 x 5 ml
145-1086 ProLine Universal Calibration Beads, 3 x 5 ml
Accessory Parts
145-1065 S3 Accessory Kit
145-1084 S3 Empty Fluidic Containers, 3 x 4 L
144-10 01 S3 Sheath Cap Assembly, white
144-10 02 S3 DI Water Cap Assembly, blue
144-10 03 S3 Waste Cap Assembly, red
144-10 05 S3 Filter Block A, empty with 2 dichroic filter holders
144-10 0 6 S3 Filter Block B, empty with 2 dichroic filter holders
144-10 07 S3 Individual Filter Holder, black, empty
144-10 08 Neutral Density Filters, 1.0
144-10 09 S3 Nozzle, 100 µm tip
144-1010 S3 Nozzle O-Rings with Alignment Disk, 2-pack
14 4-1011 S3 Collection Adaptor Set
144-1012 Hex Driver, 2 mm
144-1013 S3 Individual Dichroic Filter Holder
145-1076 S3 Power Conditioner, International
145-1077 S3 Power Conditioner, US
- Cell Sorters 1
- Legal Notices 3
- Bio-Rad Technical Support 3
- Safety Warning Labels 5
- Instrument Warning Labels 5
- Regulatory Compliance 6
- Biohazards 6
- Table of Contents 9
- Introduction 11
- 1.1 System Components 12
- 1.2 Installation Requirements 12
- Hardware Description 13
- 2.1.1 Instrument Back Panel 14
- 2.1.2 Aerosol Evacuation Port 14
- 2.2 Fluidics System 15
- 2.2.2 Loading Stage 16
- Instruction Manual 17
- 2.2.3 Nozzle 18
- 2.2.4 Sort Collection Chamber 18
- 2.3 Optics 19
- 2.4 Electronics 21
- Software 23
- 3.1 Main Software Window 24
- 3.1.1 Title Bar Buttons 25
- 3.1.2 File Menu 25
- 3.1.3 Home Tab Toolbar 26
- 3.2 Control Panel 30
- 3.3 Administrator Tab Toolbar 32
- 3.3.1 Editing QC Criteria 33
- 3.3.2 Edit Droplets 34
- 3.3.3 Edit Streams 34
- Instruction Manual 35
- Test Pattern highlighted 35
- 3.3.4 User Reports 36
- 3.3.5 Managing Users 37
- 3.3.6 Decontaminate 38
- 3.3.8 Global Preferences 39
- Boost Adjustment 40
- Force Cleaning 40
- Default Plot Statistics 40
- Plot Defaults 40
- Parameter Names 41
- Default Save Location 41
- Emergency Contact Information 41
- Getting Started 43
- 4.2 Logging In 44
- 4.3 Daily Startup 45
- 4.4 Quality Control 46
- 4.4.1 Droplet Setup 47
- 4.4.2 Side Streams 48
- 4.4.3 Alignment 49
- 4.4.5 Drop Delay Maintenance 50
- 4.4.6 Recalculate Drop Delay 51
- 4.4.7 QC Criteria 51
- 4.4.8 QC Report 51
- 4.5 Protocols and Workspace 52
- 4.5.3 Saving Protocols 53
- 4.5.5 Opening FCS Files 54
- 4.5.6 Saving FCS Files 55
- 4.5.10 Changing Parameters 56
- 4.5.11 Viewing Statistics 57
- 4.5.13 Creating Regions 58
- 4.5.14 Creating Gates 59
- Acquisition 61
- 5.1.1 Target Event Rate 62
- 5.1.2 Event Limit 63
- 5.1.3 Cycle Mode 63
- 5.1.4 Trigger 63
- 5.1.7 Auto Save 64
- 5.1.5 Threshold 64
- 5.1.6 PMT Voltages 64
- 5.2 Compensation 65
- Instruction Manual 67
- Fig. 61. Compensation matrix 67
- 5.2.2 Manual Compensation 69
- 5.3.1 Hot Swap 70
- Instruction Manual 71
- 5.4 Optical Filters 72
- 5.4.2 Optical Filter Blocks 73
- 6.1 Sort Setup 75
- Fig. 70. Sort Logic window 76
- 6.1.1 Sort Collection 77
- 6.1.2 Volume Tracker 77
- 6.1.3 Setting Regions to Sort 78
- 6.2 Sort Modes 79
- 6.3 Sort Statistics 79
- 6.4 Sort Plots 81
- Additional Software Features 83
- 7.4 Clean System 84
- 7.5 Instrument Status Box 85
- 7.6 Status Bar 85
- 7.8 Quality Control Reports 86
- 7.7 Printing 86
- 7.9 User Reports 87
- 7.10 Biosafety System 88
- Shutdown 89
- Instruction Manual 91
- 8.1 Daily Shutdown 91
- Automatic Startup 93
- 10.2 Dealing with Clogs 98
- Maintenance 100
- 10.4 Swap Nozzle Tip Wizard 101
- Instruction Manual 101
- Instruction Manual 103
- Instruction Manual 105
- Cleaning the nozzle tip: 108
- Sonication: 108
- Syringe wash: 108
- Instruction Manual 109
- Instruction Manual 111
- Instruction Manual 113
- 10.5 Optical Filter Cleaning 115
- 10.6 Disinfectants 115
- 10.7 Decontamination 115
- Instruction Manual 119
- Troubleshooting 121
- References 125
- and S3e 127
- Cell Sorter 127
- Specifications 127
- Ordering Information 129
- Life Science 132
- Bio-Rad 132
- Laboratories, Inc 132
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