Bio-rad S3™ Cell Sorter Uživatelský manuál Strana 127
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Instruction Manual | 117
13
S3
™
and S3e
™
Cell Sorter
Specifications
The specifications for the S3 and S3e Cell Sorters are shown in Table 31.
Table 31. S3 and S3e Cell Sorter specifications.
Performance Droplet frequency 37–43 kHz
Sort type True jet-in-air, high performance sorting
Sort rate No hardware limitations, limited only by droplet frequency and application
Sorting purity >99% pure
Sensitivity <125 MESF for FITC and PE
Temperature control Sample and collection temperature control from 4–37°C
Optics Lasers Single laser: 488 nm 100 mW
Single laser upgrade: 561 nm 100 mW or 640 nm 100 mW
Dual laser: 488 nm 100 mW and 561 nm 100 mW
Detection Forward scatter (FSC) with PMT
Side scatter (SSC) with PMT
4 fluorescent detectors with PMT
Fluidics Nozzle 100 µm fixed
Pressure 30 psi fixed
Sheath Onboard dilution of 8x sheath or direct use of 1x sheath
Automation Auto alignment
Auto drop delay calibration
Auto droplet monitoring
Auto stream to laser alignment
Sorting Directions Two way
Modes Single, purity, enrich
Collection Up to 5 x 5 ml or 1.5 ml sample tube each direction
Microscope slides
2 x 8 micro well strip each direction
Software Program ProSort
™
Workstation Computer requirements 5 Quad Core; 8 GB RAM; 250 GB HDD, Windows 7 Pro x64
monitor Recommended 24” LCD; 1920 x 1080 resolution
Physical Dimensions (W x D x H) 70 x 65 x 65 cm deep (instrument only)
requirements Weight 90 kg (instrument only)
Electrical 120–230 VAC; 50–60 Hz
Temperature Ambient temperature range of 18–25°C
Noise <60 dBA while in operation
Options Biosafety cabinet S3 Biosafety System Class I
Regulatory Electrical Electrical Safety for Europe
status IEC 61010-1 CB Scheme
UL/CSA Standard for Safety Electrical Equipment for
Electrical Safety (USA, Canada, NRTL)
IEC61010-2-081, EN 61010-2-081
EN/IEC 60825-1, EMC EN 61326-1
CE Declaration of Certification
Laser Class I (1) laser product per CDRH regulations and EN/IEC 60825-1
- Cell Sorters 1
- Legal Notices 3
- Bio-Rad Technical Support 3
- Safety Warning Labels 5
- Instrument Warning Labels 5
- Regulatory Compliance 6
- Biohazards 6
- Table of Contents 9
- Introduction 11
- 1.1 System Components 12
- 1.2 Installation Requirements 12
- Hardware Description 13
- 2.1.1 Instrument Back Panel 14
- 2.1.2 Aerosol Evacuation Port 14
- 2.2 Fluidics System 15
- 2.2.2 Loading Stage 16
- Instruction Manual 17
- 2.2.3 Nozzle 18
- 2.2.4 Sort Collection Chamber 18
- 2.3 Optics 19
- 2.4 Electronics 21
- Software 23
- 3.1 Main Software Window 24
- 3.1.1 Title Bar Buttons 25
- 3.1.2 File Menu 25
- 3.1.3 Home Tab Toolbar 26
- 3.2 Control Panel 30
- 3.3 Administrator Tab Toolbar 32
- 3.3.1 Editing QC Criteria 33
- 3.3.2 Edit Droplets 34
- 3.3.3 Edit Streams 34
- Instruction Manual 35
- Test Pattern highlighted 35
- 3.3.4 User Reports 36
- 3.3.5 Managing Users 37
- 3.3.6 Decontaminate 38
- 3.3.8 Global Preferences 39
- Boost Adjustment 40
- Force Cleaning 40
- Default Plot Statistics 40
- Plot Defaults 40
- Parameter Names 41
- Default Save Location 41
- Emergency Contact Information 41
- Getting Started 43
- 4.2 Logging In 44
- 4.3 Daily Startup 45
- 4.4 Quality Control 46
- 4.4.1 Droplet Setup 47
- 4.4.2 Side Streams 48
- 4.4.3 Alignment 49
- 4.4.5 Drop Delay Maintenance 50
- 4.4.6 Recalculate Drop Delay 51
- 4.4.7 QC Criteria 51
- 4.4.8 QC Report 51
- 4.5 Protocols and Workspace 52
- 4.5.3 Saving Protocols 53
- 4.5.5 Opening FCS Files 54
- 4.5.6 Saving FCS Files 55
- 4.5.10 Changing Parameters 56
- 4.5.11 Viewing Statistics 57
- 4.5.13 Creating Regions 58
- 4.5.14 Creating Gates 59
- Acquisition 61
- 5.1.1 Target Event Rate 62
- 5.1.2 Event Limit 63
- 5.1.3 Cycle Mode 63
- 5.1.4 Trigger 63
- 5.1.7 Auto Save 64
- 5.1.5 Threshold 64
- 5.1.6 PMT Voltages 64
- 5.2 Compensation 65
- Instruction Manual 67
- Fig. 61. Compensation matrix 67
- 5.2.2 Manual Compensation 69
- 5.3.1 Hot Swap 70
- Instruction Manual 71
- 5.4 Optical Filters 72
- 5.4.2 Optical Filter Blocks 73
- 6.1 Sort Setup 75
- Fig. 70. Sort Logic window 76
- 6.1.1 Sort Collection 77
- 6.1.2 Volume Tracker 77
- 6.1.3 Setting Regions to Sort 78
- 6.2 Sort Modes 79
- 6.3 Sort Statistics 79
- 6.4 Sort Plots 81
- Additional Software Features 83
- 7.4 Clean System 84
- 7.5 Instrument Status Box 85
- 7.6 Status Bar 85
- 7.8 Quality Control Reports 86
- 7.7 Printing 86
- 7.9 User Reports 87
- 7.10 Biosafety System 88
- Shutdown 89
- Instruction Manual 91
- 8.1 Daily Shutdown 91
- Automatic Startup 93
- 10.2 Dealing with Clogs 98
- Maintenance 100
- 10.4 Swap Nozzle Tip Wizard 101
- Instruction Manual 101
- Instruction Manual 103
- Instruction Manual 105
- Cleaning the nozzle tip: 108
- Sonication: 108
- Syringe wash: 108
- Instruction Manual 109
- Instruction Manual 111
- Instruction Manual 113
- 10.5 Optical Filter Cleaning 115
- 10.6 Disinfectants 115
- 10.7 Decontamination 115
- Instruction Manual 119
- Troubleshooting 121
- References 125
- and S3e 127
- Cell Sorter 127
- Specifications 127
- Ordering Information 129
- Life Science 132
- Bio-Rad 132
- Laboratories, Inc 132
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