Bio-rad ChromLab™ Software Uživatelský manuál stáhnout pdf (Strana 122)

5 | Method Editor

120 | NGC Chromatography Systems and ChromLab Software

Phase Library Pane

The Phase Library pane contains both standard and custom phases you can use to

create methods. Each phase consists of a series of programmed steps that

represent a process in chromatography. You can add or remove steps from a phase

to customize it for a specific application. You can save modified phases as custom

phases in the Custom tab where they will be available to all ChromLab software

users. Standard phases are further described in Standard Phases on page 138.

Table 4. Standard Phases

Phase Explanation

Method Settings Define the parameters used throughout the

method.

Equilibration Equilibrates the column before or after

elution.

Sample Application Applies sample to the column. Defines

mode of application, either dir

ect or through

sample loop, sample volume, flow rate, and

buffers used for sample loading.

Column Wash Washes out unbound proteins (flow

thr

ough) after sample application. When

used after an elution, the conditions defined

would remove proteins bound strongly to

the media under conditions used for

elution.

Elution Elutes the sample from the column.

System CIP (Clean in Place)/

Storage

Rinses the system with a cleaning solution.

This phase pulls fr

om a single cleaning or

storage solution.

Note: System CIP must be run as the

only type of phase in the method. Y

can add several System CIP phases to

the method if multiple cleaning

solutions are required.

1 2 ... 117 118 119 120 121 122 123 124 125 126 127 ... 299 300

Žádné komentáře

Bio-rad ChromLab™ Software Uživatelský manuál Strana 122