Bio-rad ChromLab™ Software Uživatelský manuál stáhnout pdf (Strana 44)

2 | The NGC Instrument

42 | NGC Chromatography Systems and ChromLab Software

Buffer Blending Valve

The buffer blending valve is used for automatic online buffer preparation. The buffer

blending valve blends buffers to a specific pH using standard acid and base buffer

solutions. This enables automated pH scouting without manually preparing buffers

at various pH values. The buffer blending valve titrates the appropriate amounts of

the acid and base stock solutions to produce buffers at the required pH values.

Because salt concentration can affect pH, the blending algorithm takes into account

the salt concentration for accurate pH blending.

Stock solutions of four buffers are connected to the inlets. The buffer blending valve

can be connected dir

ectly into the inlet of the system pumps or to port 8/BB on the

buf

fer inlet valves. In addition to buffer blending, the buffer blending valve can be

used to double the allowed flow rate of a salt gradient (for example, up to 20 ml/min

on an F10 system pump and 200 ml/min on an F100 system pump).

The buffer blending valve can be plumbed to two buffer inlet valves, each

connected to seven dif

ferent buffers. The system can switch between blending

mode and simple salt gradients without replumbing.

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Bio-rad ChromLab™ Software Uživatelský manuál Strana 44