Bio-rad ChromLab™ Software Uživatelský manuál stáhnout pdf (Strana 124)

3 | Preparing the Instrument

122 | NGC Chromatography Systems and ChromLab Software

Priming and Purging the NGC Scout System

Priming the NGC Scout system is a manual procedure and involves priming the

buffer blending valve starting from the Q4 port to Q1. You can purge one line at a

time, or all lines sequentially. These procedures require access to ChromLab.

Tip: You can perform the steps on the NGC touch screen.

Priming the NGC Scout System

Note: This section assumes that you have not introduced buffer into the lines.

If you have buffer in the lines and are purging the lines of small air bubbles,

proceed to Purging the NGC Scout System on page 124.

To prime the buffer blending valve

1. Immerse the inlet tubing for ports Q1–Q4 in their respective buffer containers.

(See To plumb the buffer blending valve on page 276 for more information.)

2. On the ChromLab computer or the touch screen, start ChromLab in Manual

mode.

3. Set the fluidic scheme to either NGC Scout or NGC Scout Plus.

4. In the fluidic scheme, touch or click the System Pump module to open its

dialog box.

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Bio-rad ChromLab™ Software Uživatelský manuál Strana 124