Bio-rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit Uživatelský manuál stáhnout pdf (Strana 7)

Section 3

Storage Conditions

Store components of the kit at the recommended temperatures (see Table 1

below).

Table 1. Recommended storage temperature for the Aurum™ total

RNA fatty and fibrous tissue kit components.

Kit Components Storage Temperature

PureZOL™ RNA isolation reagent Store at 4–25°C

Low stringency wash buffer Room temperature (before and after the

addition of ethanol)

High stringency wash buffer Room temperature

Elution solution Room temperature

DNase dilution solution 4°C; allow solution to equilibrate to

room temperature before use

DNase I (lyophilized) Room temperature in lyophilized form;

once reconstituted in 250 µl of 10 mM

Tris, pH 7.5, aliquot and store at –20°C

in a nonfrost-free freezer. Do not freeze-

thaw more than once after

reconstitution

Aurum RNA binding mini columns Room temperature

and microcentrifuge tubes

Section 4

Materials and Equipment Required

(Not Provided in the Kit)

• Microcentrifuge, capable of spinning at >12,000 x g at 4°C and room

temperature

• 95–100% ethanol, ACS grade or better

• Tris for DNase I reconstitution (catalog #161-0716)

• Chloroform (without additives such as isoamyl alcohol) for phase

separation; 0.2 ml of chloroform per 1 ml of PureZOL required

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Bio-rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit Uživatelský manuál Strana 7