Bio-rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit Uživatelský manuál stáhnout pdf (Strana 14)

Table 3. Disruption and homogenization methods.

Starting

Material Disruption Method Homogenization Method

Animal tissue Grind tissue with a Pipetting up and down,

mortar and pestle rotor-stator homogenizer,

under liquid nitrogen, bead mill homogenizer, or

use a rotor-stator 18-gauge needle and syringe

homogenizer, bead

mill homogenizer, or

18-gauge needle

and syringe

Plant tissue Grind tissue with a Pipetting up and down,

mortar and pestle rotor-stator homogenizer,

under liquid nitrogen, bead mill homogenizer, or

use a rotor-stator 18-gauge needle and syringe

homogenizer, bead

mill homogenizer, or

18-gauge needle

and syringe

Cultured cells Pipet up and Pipetting up and down, or

down, or use 18-gauge 18-gauge needle and syringe

needle and syringe

Bacteria Rotor-stator Pipetting up and down,

homogenizer, bead rotor-stator homogenizer, or

mill homogenizer, bead mill homogenizer

pipet up and down,

or 18-gauge needle

and syringe

Yeast Rotor-stator Pipetting up and down,

homogenizer, bead rotor-stator homogenizer, or

mill homogenizer, or bead mill homogenizer

18-gauge needle

and syringe

• Mortar and pestle: freeze the tissue with liquid nitrogen, then grind it into a

fine powder under liquid nitrogen

• Pipet up and down: pass the lysate through a standard micropipet tip

several times

• 18-gauge needle and syringe: pass the lysate through the needle several

times

• Rotor-stator homogenizer: immerse the tip of the homogenizer into the

solution and homogenize for 30–60 sec

• For bead mill homogenizers, follow manufacturer's instructions

If column clogging occurs, switching to a more vigorous homogenization

method may lower the incidence of column clogging.

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