Bio-RadProtein AssayFor Technical ServiceCall Your Local Bio-Rad Office orin the U.S. Call 1-800-4BIORAD(1-800-424-6723)LIT33C 8/31/98 02:25 PM Page
8Section 3Common Questions1. The buffer that I normally use is not listed in the reagent compatibilitylist. How will I know if it interferes with the
94. My sample is a mixture of proteins. Which protein standard should Iuse to generate the standard curve?In any protein assay, the best protein to u
Table 2. Comparison of Bio-Rad, Lowry, and Biuret ProteinAssays for 23 ProteinsAll assays were performed on appropriate dilutions of gravimetrically
116. Is any sample preparation required?In general, no. However, the protein must be solubilized. (The samplecan not be a suspension or an unfiltered
12Section 4Troubleshooting Guide1. How can protein with a typical low dye response be assayed?Occasionally a protein will be assayed which yields exce
13Section 5Ordering InformationCatalog Number Description500-0001 Bio-Rad Protein Assay Kit I, includes 450 ml dye reagentconcentrate and lyophilized
14Section 6Safety InformationMATERIAL SAFETY DATA SHEETI. PRODUCT IDENTIFICATIONTRADE NAME: Methanol (as used in Protein Assay Kits I and II andDye Re
15III. PHYSICAL/CHEMICAL CHARACTERISTICS BOILING POINT: 64.5 ˚C (148 ˚F)VAPOR PRESSURE: 97 mm Hg at 20 ˚CEVAPORATION RATE (BUTYL ACETATE = 1): 5.9SOLU
16ABSORBED THROUGH SKIN: May be absorbed through intact skinand produce systemic effects.SWALLOWED: Can cause gastrointestinal irritation, headache, n
17SUSPECTED CANCER AGENT X NO: THIS PRODUCT’S INGREDIENTS ARE NOT FOUND INTHE LISTS BELOW.YES: _____FEDERAL OSHA ______NTP ______IARCMEDICAL CON
Table of ContentsSection 1 Introduction... 11.1 Principle ...
18VIII. SPECIAL HANDLING INFORMATION VENTILATION AND ENGINEERING CONTROLS: Provide suffi-cient mechanical explosion-proof ventilation to maintain expo
19MATERIAL SAFETY DATA SHEETI. PRODUCT IDENTIFICATIONTRADE NAME: Phosphoric Acid (as used in Protein Assay Kits I & IIand the Dye Reagent Concentr
20III. PHYSICAL/CHEMICAL CHARACTERISTICSBOILING POINT: l58 ˚C (3l6 ˚F)VAPOR PRESSURE: 0.03 mm Hg at 20 ˚CEVAPORATION RATE (BUTYL ACETATE = 1): N/ASOLU
21SWALLOWED: Corrosive; may cause sore throat, abdominal pain, nau-sea, and severe burns.HEALTH EFFECTS OR RISKS FROM EXPOSUREACUTE: Phosphoric acid i
22SUSPECTED CANCER AGENT x NO: THIS PRODUCT’S INGREDIENTS ARE NOT FOUND INTHE LISTS BELOW.YES: _____FEDERAL OSHA ______NTP ______IARCMEDICAL CON
23VIII. SPECIAL HANDLING INFORMATION VENTILATION AND ENGINEERING CONTROLS: A local exhaustsystem is recommended to maintain levels below exposure limi
24Section 7References1. Bradford, M., Anal. Biochem., 72, 248 (1976).2. Reisner, A. H., Nemes, P. and Bucholtz, C., Anal. Biochem., 64, 509 (1975).3.
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1Section 1IntroductionThe Bio-Rad Protein Assay, based on the method of Bradford, is asimple and accurate procedure for determining concentration of s
2Table 1. Reagents Compatible with the Bio-Rad Protein AssayWhen Using the Standard Procedure.*Acetate, 0.6 M KCI, 1.0 MAcetone Malic acid, 0.2 MAdeno
31.2 Product DescriptionProtein Assay Dye Reagent Concentrate (catalog number 500-0006)contains 450 ml of solution containing dye, phosphoric acid, a
4Section 2Instructions2.1 Reconstituting the StandardTo reconstitute the lyophilized bovine gamma globulin and bovineserum abumin standards, add 20 m
5Fig. 1. Typical standard curve for the Bio-Rad Protein Assay, bovine gammaglobulin (standard I), bovine serum albumin (standard II). O.D.595 correcte
6Fig. 2. Typical standard curve for the Bio-Rad Protein Microassay (1-20 µg/ml),bovine gamma globulin (standard I), bovine serum albumin (standard II)
73. Pipet 10 µl of each standard and sample solution into separatemicrotiter plate wells. 4. Add 200 µl of diluted dye reagent to each well. Mix the s
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