Bio-rad Protein Assay Uživatelský manuál

Procházejte online nebo si stáhněte Uživatelský manuál pro Měřící nástroje Bio-rad Protein Assay. Bio-Rad Protein Assay User Manual Uživatelská příručka

  • Stažení
  • Přidat do mých příruček
  • Tisk
  • Strana
    / 27
  • Tabulka s obsahem
  • ŘEŠENÍ PROBLÉMŮ
  • KNIHY
  • Hodnocené. / 5. Na základě hodnocení zákazníků
Zobrazit stránku 0
Bio-Rad
Protein Assay
For Technical Service
Call Your Local Bio-Rad Office or
in the U.S. Call 1-800-4BIORAD
(1-800-424-6723)
LIT33C 8/31/98 02:25 PM Page a
Zobrazit stránku 0
1 2 3 4 5 6 ... 26 27

Shrnutí obsahu

Strany 1 - Protein Assay

Bio-RadProtein AssayFor Technical ServiceCall Your Local Bio-Rad Office orin the U.S. Call 1-800-4BIORAD(1-800-424-6723)LIT33C 8/31/98 02:25 PM Page

Strany 2 - Table of Contents

8Section 3Common Questions1. The buffer that I normally use is not listed in the reagent compatibilitylist. How will I know if it interferes with the

Strany 3 - Introduction

94. My sample is a mixture of proteins. Which protein standard should Iuse to generate the standard curve?In any protein assay, the best protein to u

Strany 4

Table 2. Comparison of Bio-Rad, Lowry, and Biuret ProteinAssays for 23 ProteinsAll assays were performed on appropriate dilutions of gravimetrically

Strany 5 - 1.2 Product Description

116. Is any sample preparation required?In general, no. However, the protein must be solubilized. (The samplecan not be a suspension or an unfiltered

Strany 6 - Instructions

12Section 4Troubleshooting Guide1. How can protein with a typical low dye response be assayed?Occasionally a protein will be assayed which yields exce

Strany 7 - 2.3 Microassay Procedure

13Section 5Ordering InformationCatalog Number Description500-0001 Bio-Rad Protein Assay Kit I, includes 450 ml dye reagentconcentrate and lyophilized

Strany 8

14Section 6Safety InformationMATERIAL SAFETY DATA SHEETI. PRODUCT IDENTIFICATIONTRADE NAME: Methanol (as used in Protein Assay Kits I and II andDye Re

Strany 9

15III. PHYSICAL/CHEMICAL CHARACTERISTICS BOILING POINT: 64.5 ˚C (148 ˚F)VAPOR PRESSURE: 97 mm Hg at 20 ˚CEVAPORATION RATE (BUTYL ACETATE = 1): 5.9SOLU

Strany 10 - Common Questions

16ABSORBED THROUGH SKIN: May be absorbed through intact skinand produce systemic effects.SWALLOWED: Can cause gastrointestinal irritation, headache, n

Strany 11

17SUSPECTED CANCER AGENT X NO: THIS PRODUCT’S INGREDIENTS ARE NOT FOUND INTHE LISTS BELOW.YES: _____FEDERAL OSHA ______NTP ______IARCMEDICAL CON

Strany 12 - Assays for 23 Proteins

Table of ContentsSection 1 Introduction... 11.1 Principle ...

Strany 13

18VIII. SPECIAL HANDLING INFORMATION VENTILATION AND ENGINEERING CONTROLS: Provide suffi-cient mechanical explosion-proof ventilation to maintain expo

Strany 14 - Troubleshooting Guide

19MATERIAL SAFETY DATA SHEETI. PRODUCT IDENTIFICATIONTRADE NAME: Phosphoric Acid (as used in Protein Assay Kits I & IIand the Dye Reagent Concentr

Strany 15 - Ordering Information

20III. PHYSICAL/CHEMICAL CHARACTERISTICSBOILING POINT: l58 ˚C (3l6 ˚F)VAPOR PRESSURE: 0.03 mm Hg at 20 ˚CEVAPORATION RATE (BUTYL ACETATE = 1): N/ASOLU

Strany 16 - Section 6

21SWALLOWED: Corrosive; may cause sore throat, abdominal pain, nau-sea, and severe burns.HEALTH EFFECTS OR RISKS FROM EXPOSUREACUTE: Phosphoric acid i

Strany 17 - V. HEALTH HAZARD INFORMATION

22SUSPECTED CANCER AGENT x NO: THIS PRODUCT’S INGREDIENTS ARE NOT FOUND INTHE LISTS BELOW.YES: _____FEDERAL OSHA ______NTP ______IARCMEDICAL CON

Strany 18

23VIII. SPECIAL HANDLING INFORMATION VENTILATION AND ENGINEERING CONTROLS: A local exhaustsystem is recommended to maintain levels below exposure limi

Strany 19 - VI. REACTIVITY DATA

24Section 7References1. Bradford, M., Anal. Biochem., 72, 248 (1976).2. Reisner, A. H., Nemes, P. and Bucholtz, C., Anal. Biochem., 64, 509 (1975).3.

Strany 20

U.S. (800) 4BIORAD • California Ph. (510) 741-1000 • New York Ph. (516) 756-2575 • Australia Ph. 02-805-5000 • Austria Ph. (1) 877 89 01 • Belgium

Strany 21

1Section 1IntroductionThe Bio-Rad Protein Assay, based on the method of Bradford, is asimple and accurate procedure for determining concentration of s

Strany 22

2Table 1. Reagents Compatible with the Bio-Rad Protein AssayWhen Using the Standard Procedure.*Acetate, 0.6 M KCI, 1.0 MAcetone Malic acid, 0.2 MAdeno

Strany 23

31.2 Product DescriptionProtein Assay Dye Reagent Concentrate (catalog number 500-0006)contains 450 ml of solution containing dye, phosphoric acid, a

Strany 24

4Section 2Instructions2.1 Reconstituting the StandardTo reconstitute the lyophilized bovine gamma globulin and bovineserum abumin standards, add 20 m

Strany 25

5Fig. 1. Typical standard curve for the Bio-Rad Protein Assay, bovine gammaglobulin (standard I), bovine serum albumin (standard II). O.D.595 correcte

Strany 26 - References

6Fig. 2. Typical standard curve for the Bio-Rad Protein Microassay (1-20 µg/ml),bovine gamma globulin (standard I), bovine serum albumin (standard II)

Strany 27 - Life Science

73. Pipet 10 µl of each standard and sample solution into separatemicrotiter plate wells. 4. Add 200 µl of diluted dye reagent to each well. Mix the s

Komentáře k této Příručce

Žádné komentáře