Bio-rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit Uživatelský manuál stáhnout pdf (Strana 29)

Section 9

Troubleshooting Guide

Problems that may be encountered during RNA purification:

Problem Possible Cause Recommended Solution

Incomplete Lysate was not mixed Once chloroform is

separation of properly after adding added, mix tubes

phases after chloroform (see step 4 vigorously by shaking

centrifugation in protocol) for 15 sec. Do not vortex!

Let the lysate incubate for

5 min at room

temperature, mix again

before centrifuging

Lysate was not Make sure that

centrifuged at the right centrifugation step is

temperature performed at 4°C

following the addition of

chloroform in order to

achieve complete

separation of the phases

Incorrect amount of For every 1 ml of PureZOL™

chloroform was added used, add 0.2 ml of

chloroform

RNA binding mini Incomplete disruption of Increase the duration or

column is clogging starting material intensity of sample

disruption. Make sure to

use the 1 ml of PureZOL

for each prep

Excessive amount of Do not exceed the

starting material maximum starting amount

limit for the kit (see

Section 5). If clogging

persists when using the

maximum starting

amount, reduce the

amount of material used

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Bio-rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit Uživatelský manuál Strana 29