Bio-rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit Uživatelský manuál stáhnout pdf (Strana 31)

Problem Possible Cause Recommended Solution

Low RNA yield Low amount of Do not use less than the

(continued) starting material recommended minimum

starting amount (see

Section 5). When

processing small

amounts of starting

material (<500,000 cells

or <10 mg of tissue),

perform a single elution

with 30 µl of elution

solution

Incomplete disruption Increase the duration or

of starting material intensity of sample

that causes cells not disruption. Make sure

to be lysed, and thus that 1 ml of PureZOL is

fail to release RNA used per prep

into the lysate to be

recovered

Incorrect use of wash Add the required amount

solutions. (Incorrect of ethanol to the

ethanol concentration low stringency wash

in the low stringency buffer before use

wash can cause

accidental elution of

the RNA from the

membrane)

Incorrect DNase I Use only the DNase

dilution solution used, dilution solution included

causing accidental in the kit to dilute the

elution of the RNA DNase I

from the membrane

Elution solution was Avoid pipetting the

not pipetted directly elution solution onto the

onto the center of the side of the column or on

membrane top of the ring that holds

the membrane stack in

place

Elution contamination Prior to eluting the RNA,

of the eluate make sure to perform the

purge spin step (see step

16 in spin protocol) to

remove residual ethanol

in the wash solution

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