Bio-rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep Uživatelský manuál stáhnout pdf (Strana 21)

Table 4. Common buffers for ion exchange

chromatography.

Type of Buffering Buffer Range

Cation Exchanger

Acetic acid 4.8–5.2

Citric acid 4.2–5.2

HEPES 7.6–8.2

Lactic acid 3.6–4.3

MES 5.5–6.7

MOPS 6.5–7.9

Phosphate 6.7–7.6

PIPES 6.1–7.5

Pivalic acid 4.7–5.4

TES 7.2–7.8

Tricine 7.8–8.9

Anion Exchanger

Bicine 7.6–9.0

Bis-Tris 5.8–7.2

Diethanolamine 8.4–8.8

Diethylamine 9.5–11.5

L-Histidine 5.5–6.0

Imidazole 6.6–7.1

Pyridine 4.9–5.6

Tricine 7.8–8.9

Triethanolamine 7.3–8.0

Tris 7.5–8.0

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