Bio-rad Chromatography Standards Uživatelský manuál stáhnout pdf

Gel Filtration

Standard

Instruction Manual

Catalog #

151-1901

Lit102E.qxd 09/13/2002 2:35 PM Page A

1 2 3 4 5 6 7 8

Shrnutí obsahu

Strany 1 - Standard

Gel FiltrationStandardInstruction ManualCatalog #151-1901Lit102E.qxd 09/13/2002 2:35 PM Page A

Strany 2 - Table of Contents

Table of ContentsSection 1 Introduction... 11.1 Instructions...

Strany 3 - Introduction

Section 1IntroductionBio-Rad’s gel filtration standard is a lyophilized mixture of molecularweight markers ranging from 1,350 to 670,000 daltons. It i

Strany 4 - 25–50 ml 0.125 ml

1.2 Recommended Volume of StandardThe general recommendation is to load no more than 1% of the column volume to avoid peak broadening effects. To achi

Strany 5

3Eluent volumea. Bio-Sil SEC 125 columnEluent volumeb. Bio-Sil SEC 250 columnEluent volumec. Bio-Sil SEC 400 columnFig. 1. Separation of standard on H

Strany 6

Fig. 1. a. Bio-Sil SEC 125 column (exclusion limit* ≈ 80,000).b. Bio-Sil SEC 250 column (exclusion limit ≈ 300,000).c. Bio-Sil SEC 400 column (exclusi

Strany 7 - Section 2

51.3 Shelf LifeThe lyophilized standard is stable for at least 1 year when stored at 2 to 8°C. The shelf life may be extended if stored at a lower tem

Strany 8 - LIT102 Rev E

Bio-Rad Laboratories, Inc.2000 Alfred Nobel Dr., Hercules, CA 94547 USA510-741-1000LIT102 Rev ELit102E.qxd 09/13/2002 2:35 PM Page 6