Bio-rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep Uživatelský manuál Strana 1

Bio-Scale™Mini Macro-Prep®High Q, High S and DEAECartridges, 1 and 5 mlInstruction ManualCatalog #732-4120, 732-4122, 732-4124,732-4130, 732-4132, 732

Assemble with fittings and lock rings as shown inFigure 1.(Use orange lock rings and medium size barb fittings with 1.6 mm tubing.)Fig. 1. BioLogic L

2. To maximize gradient accuracy and to applysamples efficiently, install 1.6 mm ID tubingfrom the pump to the MV-6 sample inject valve(if available)

3. Connect the inlet of the cartridge to the maleluer fitting on the MV-6 sample inject valve (seeFigure 2). If not using the MV-6 sample injectvalve

Fig. 3. Cartridge and fittings.910006400B.qxp 7/10/2006 3:48 PM Page 9

Section 3Connecting to Other LiquidChromatography SystemsBio-Scale Mini cartridges can be connected to anyliquid chromatography system, provided that

Fig. 4. Luer to 1/4-28 adaptor.3.2 HPLC SystemsThe luer to 10-32 adaptor fittings kit (catalog #732-0112) provides fittings necessary to connectthe

3.3 FPLC SystemsThe luer to M6 adaptor fittings kit (catalog #732-0111)provides fittings necessary to connect the Bio-ScaleMini cartridge to the M6 f

Section 4Preparing a Cartridge For UseBio-Scale Mini Macro-Prep High Q, Macro-PrepHigh S, and Macro-Prep DEAE cartridges contain20% ethanol (v/v) as t

4. Equilibrate the cartridge with low-salt buffer for5 min.5. Reduce the flow rate to that which will be usedin the purification protocol.4.1 Sampl

Table 3. Products for buffer exchange.Sample RecommendedVolume Product Use Catalog #50–100 µl Bio-Spin 6 column Desalting proteins 732-6002≥ 6 kD5

gradient from 0.0 to 0.4 M NaCl spanning 1 to 20column volumes at 120 cm/hr, or 0.5 ml/min for the1 ml cartridge and 2.5 ml/min for the 5 ml cartridge

Table 4. Common buffers for ion exchangechromatography.Type of Buffering Buffer RangeCation ExchangerAcetic acid 4.8–5.2Citric acid 4.2–5.2HEPES 7.6

Section 5Care of the Cartridge5.1 RegenerationAfter each use, the cartridge should be regeneratedwith the appropriate salt, in most cases 1–2 M NaCli

appropriate cleaning protocol. As a general guide, werecommend the following:1. Use high salt buffer for regeneration, as above.2. For aggregated or

is equilibrated in the starting buffer before loading the sample. 5.3 StorageAfter washing the cartridges with deionized water,Bio-Scale Mini ion exc

Section 7Ordering InformationBio-Scale Mini Cartridges*Description 5 x 1 ml 1 x 5 ml 5 x 5 mlUNOsphere™Q Support 732-4100 731-4102 731-4104UNOsphere

Catalog # DescriptionFittings Kits732-0111 Luer to M6 Adaptor Fittings Kit, includes luer toM6 fitting to connect to an FPLC system732-0112 Luer to

Section 8References1. Gagnon P, Avoiding Instrument-associatedAberrations in Purification Scale-up and Scale-down, BioPharm 10, 42–45 (1997)2. Harri

Bio-Rad Laboratories, Inc.2000 Alfred Nobel Dr.Hercules, CA 94547 USA510-741-10001-800-424-672310006400 Rev C 10006400B.qxp 7/10/2006 3:48 PM

Table of ContentsSection 1...Introduction ...1Section 2 Connecting to Bio-Rad’sLow-Pressure ChromatographyInstrum

5.1 Regeneration ...185.2 Cleaning...185.3 Storage...20Section 6 Te

Section 1IntroductionBio-Scale Mini cartridges have a patent-pendingdouble-wall design that provides extra durability andallows easy, reliable runs wi

Bio-Scale Mini Macro-Prep High Q and S and Macro-Prep DEAE cartridges are packed withMacro-Prep®ion exchange media. These media arebased on hydrophili

Table 1. Bio-Scale Mini Macro-Prep High Q,High S, and DEAE cartridge specifications.Sizes 1 ml and 5 ml bed volumesDimensions1 ml: 40 mm length x 5.

Table 2. Macro-Prep High Q & S and Macro-Prep DEAE specifications.Properties High Q High S DEAEType of ion exchanger Strong anion Strong cation

Section 2Connecting to Bio-Rad’sLow-PressureChromatographyInstrumentsBio-Scale Mini cartridges are ideal for use with Bio-Rad's BioLogic™LP chrom