Bio-rad Aurum™ Total RNA Mini Kit Uživatelský manuál stáhnout pdf (Strana 8)

Table 1. Yield (per column) of total RNA from various samples

using the Aurum total RNA mini kit.

Starting Material Avg. Yield (µg)*

Cultured cells (2 x 10

)

3T3 20–30

HeLa 20–40

Bacteria (2.4 x 10

)

E. coli 30–35

B. cereus 30–35

Yeast (3 x 10

)

S. cerevisiae 20–25

Animal tissue (up to 40 mg)

Brain 15–20

Liver 8–12

Lung 8–12

Kidney 15–20

Spleen 15–25

Plant tissue (up to 60 mg)

Arabidopsis 5–10

Maize 5–10

Potato 15–20

Spinach 10–15

Tomato 5–10

Tobacco 5–10

Starting material amounts in parentheses are the maximum amounts recommended for use with the

total RNA mini kit.

*Yield figures are representative of a minimum of 20 mini column preps performed in both

vacuum and spin formats.

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Bio-rad Aurum™ Total RNA Mini Kit Uživatelský manuál Strana 8