Bio-rad Aurum™ Total RNA Mini Kit Uživatelský manuál stáhnout pdf (Strana 11)

Table 2. Disruption and homogenization methods.

Starting Disruption Homogenization

Material Method Method

Cultured mammalian Lysis solution Pipetting up and down

cells 18-gauge needle and syringe

Bacteria Lysozyme Pipetting up and down

+ lysis solution 18-gauge needle and syringe

Yeast Lyticase Pipetting up and down

+ lysis solution 18-gauge needle and syringe

Animal tissue Mortar and pestle Rotor-stator homogenizer*

+ lysis solution Pipetting up and down

18-gauge needle and syringe

Plant tissue Mortar and pestle Rotor-stator homogenizer*

+ lysis solution Pipetting up and down

18-gauge needle and syringe

*Rotor-stator homogenizers are recommended for animal and plant tissue.

• Mortar and pestle: freeze the tissue with liquid nitrogen, then grind it into a

fine powder under liquid nitrogen

• Pipetting up and down: pass the lysate through a standard micropipettor tip

several times

• 18-gauge needle and syringe: pass the lysate through the needle several

times

• Rotor-stator homogenizer: immerse the tip of the homogenizer into the

solution and homogenize for 30–60 sec

If column clogging occurs, switching to a more vigorous homogenization

method may lower the incidence of column clogging

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Bio-rad Aurum™ Total RNA Mini Kit Uživatelský manuál Strana 11