Bio-rad Aurum™ Total RNA Mini Kit Uživatelský manuál stáhnout pdf (Strana 6)

Section 3

Storage Conditions

All kit components (including lyophilized DNase I) should be stored at room

temperature. Store reconstituted DNase I at –20°C in a nonfrost-free freezer,

avoiding repeated freeze-thaw cycles. If precipitation is observed in any

solution, warm the solution to 37°C to redissolve and allow the solution to

return to room temperature before use.

Section 4

Necessary Supplies

Equipment and reagents to be provided by the customer:

• Microcentrifuge (>12,000 x g)

• b-mercaptoethanol, 14.2 M (catalog # 161-0710)

• Lyticase (for yeast RNA isolation only)

• Lysozyme (for bacterial RNA isolation only)

• Isopropanol (for bacterial RNA isolation only)

• Polyvinylpyrrolidone-40 (PVP), 2% (for plant isolation only)

• 95–100% ethanol

• Tris for DNase I reconstitution (catalog # 161-0716)

Additional equipment required for vacuum format:

• Aurum™ vacuum manifold with vacuum regulator and column adaptor plate

(catalog # 732-6470), or other vacuum manifold with luer fittings

• Vacuum source (capability of –23 inHg required)

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Bio-rad Aurum™ Total RNA Mini Kit Uživatelský manuál Strana 6