Bio-rad Quantum Prep Plasmid Kits Uživatelský manuál stáhnout pdf (Strana 6)

Section 3

References

1. U.S. Patent 5,075,430 issued to Bio-Rad Laboratories.

2. Ausubel et al., Current Protocols in Molecular Biology,

Wiley-Interscience, New York (1987).

Section 4

Product Information

Catalog

Number Product Description

732-6137 Quantum Prep MaxiPrep Spin Baskets, 10

732-6134 Quantum Prep Maxi/Midiprep Wash Buffer,

270 ml

732-6120 Quantum Prep Plasmid Midiprep Kit, 20 preps

732-6122 Quantum Prep Neutralization Solution, 110 ml

732-6116 Quantum Prep Midi Spin Columns, 20 with caps

732-6024 Quantum Prep Wash Buffer, 250 ml

732-6100 Quantum Prep Plasmid Miniprep Kit, 100 preps

732-6110 Quantum Prep Matrix, 20 ml

11. Remove a spin column from the bag and snap off the

tab at the tip. Place the spin column inside a 2 ml col-

lection tube. Transfer the resuspended matrix from

the previous step to the spin column. Puncture a

hole in a spin column cap with a small, sharp object

and cap the column with it. Spin 30 seconds in a

microcentrifuge at 12–14,000 x g. Remove the spin

column from its microcentrifuge tube, discard the

wash buffer at the bottom of the tube and replace

the filter in the same tube.

12. Add 500 µl of wash buffer and spin 30 seconds in a

microcentrifuge at 12–14,000g. Remove spin column

and discard Wash Buffer. If isolating DNA from an

EndA+ strain do two additional 500 µl wash steps.

13. Replace the spin column in the tube and spin an

additional 2 minutes at maximum speed to remove

any residual wash buffer.

14. Transfer the spin column to a clean 2 ml microcen-

trifuge tube. Add 600 µl of water or TE to the matrix.

Spin for 2 minutes in a microcentrifuge and discard the

spin column containing the matrix. Store plasmid

DNA at -20 °C.

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Bio-rad Quantum Prep Plasmid Kits Uživatelský manuál Strana 6