Bio-rad Profinity eXact™ Purification and Tag Cleavage Con Uživatelský manuál stáhnout pdf (Strana 2)

Table of Contents

Section 1...Introduction ........................................1

Section 2 Product Information............................2

Section 3 Connection to Low-Pressure

Chromatography Systems..................7

Section 4 Connection to Medium- and High-

Pressure Chromatography Systems .11

4.1 BioLogic DuoFlow

™

Systems .................................11

4.2 HPLC Systems .......................12

4.3 FPLC Systems........................13

Section 5...Buffers and Methods........................14

Section 6 Quick Solubility Screening

Protocol............................................16

Section 7 Preparation of E. coli Lysate .............19

Section 8 Cartridge Preparation and

Purification Protocol .........................20

Bio-Rad Laboratories, Inc.

2000 Alfred Nobel Dr.

Hercules, CA 94547 USA

510-741-1000

1-800-424-6723

10011164 Rev B

10011164A.qxp 11/19/2007 9:54 AM Page II

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Bio-rad Profinity eXact™ Purification and Tag Cleavage Con Uživatelský manuál Strana 2