Bio-rad Aurum™ Total RNA 96 Kit Uživatelský manuál stáhnout pdf (Strana 6)

Section 3

Storage Conditions

All kit components (including lyophilized DNase I) should be stored at

room temperature. Store reconstituted DNase I at –20°C in a nonfrost-

free freezer, avoiding repeated freeze-thaw cycles. If precipitation is

observed in any solution, warm the solution to 37°C to redissolve and

allow the solution to return to room temperature before use.

Section 4

Necessary Supplies

Equipment and supplies to be provided by the customer:

 • Platecentrifuge,capableofgenerating1,500xg

 • b-mercaptoethanol, 14.2 M (catalog # 161-0710)

 • Lyticase(foryeastRNAisolationonly)

 • Lysozyme(forbacterialRNAisolationonly)

 • Isopropanol(forbacterialRNAisolationonly)

 • 95–100%ethanol

 • TrisforDNaseIreconstitution(catalog#161-0716)

 • Multichannelpipettor(recommended)

 • Reagentreservoirs(recommended)

 • Additionalsealingtape(catalog#MSB-1001)(optional)

Additional equipment required for vaccum format:

 • Aurum™VacuumManifoldwithregulator(catalog#732-6470)

 • Vacuumsource(capabilityof–23inHgorapproximately–800mbar

required)

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Bio-rad Aurum™ Total RNA 96 Kit Uživatelský manuál Strana 6