Bio-rad Aurum™ Total RNA 96 Kit Uživatelský manuál stáhnout pdf (Strana 19)

Section 9

Troubleshooting Guide

Problem Possible Cause Recommended Solution

Difficulty achieving Purge step in protocol If sealing of wells with

–17 to –23 inHg sealing tape restores

negative pressure negative pressure, no

corrective action is

required; however, do

not leave sealed

   Openvacuumregulator Turncontrolknobfully

clockwise to close

regulator

Plate not seating on Press down gently on

vacuum manifold RNA binding plate to

seat

Manifold top not seated Ensure that manifold

properly on base top is seated properly on

base

Residue on gasket Rinse manifold and

gasket with distilled

water and wipe dry

Gasket detached from Press down to

manifold reestablish uniform

adhesive contact or

replace gasket

Worn manifold gasket Replace gasket

Cross-contamination Abrupt application of Increase negative

between adjacent negative pressure during pressure gradually

wells elution over 5–10 sec using

the vacuum regulator

   Residualwashbufferon BlotRNAbindingplate

drip directors drip directors with paper

towels; ensure that the

plate underside is dry

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Bio-rad Aurum™ Total RNA 96 Kit Uživatelský manuál Strana 19