Bio-rad SmartSpec Plus Spectrophotometer Uživatelský manuál stáhnout pdf (Strana 50)

7.5 OD600

This assay is useful for monitoring growth of bacterial cultures. When you press OD600,

SmartSpec Plus will display the current conversion factor and offer you the choice of

accepting or modifying it.

• If the displayed conversion factor is acceptable, press Enter. Otherwise, press

Select to toggle from Yes to No and press Enter.

• Use the number keys and the exp key to input the new conversion factor and press

Enter.

SmartSpec Plus is now ready to collect OD600 readings. For best results, you should

first zero the SmartSpec Plus by inserting a cuvette with media (without cells) and

pressing Read Blank.

Cells/ml:

Cells/ml = A600 * Conversion Factor * dilution factor

Default conversion factor is 5 x 10

cells/ml

A600 1.0=xx.xexx cell/ml

Is this factor OK? Yes

Enter new conversion

factor; xx.xexx cell/ml

Ready to read absorbance

<=Exit Assay >=Options

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Bio-rad SmartSpec Plus Spectrophotometer Uživatelský manuál Strana 50