Bio-rad RC DC™ Protein Assay Uživatelský manuál stáhnout pdf (Strana 1)

RC DC Protein Assay

Instruction Manual

Catalog # 500-0119

500-0120

500-0121

500-0122

For Technical Service

Call Your Local Bio-Rad Office or

in the U.S. Call 1-800-4BIORAD

(1-800-424-6723)

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Strany 1 - (1-800-424-6723)

RC DC Protein AssayInstruction Manual Catalog # 500-0119500-0120500-0121500-0122For Technical ServiceCall Your Local Bio-Rad Office orin the U.S. Call

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Bio-Rad Laboratories2000 Alfred Nobel Dr., Hercules, CA 94547510-741-1000 4110107 Rev A4110107A.qxd 08/29/2000 11:38 AM Page Covr2

Strany 3 - Section 4 Assay Instructions

1Section 1 IntroductionThe RC DC Protein Assay is a colorimetric assay for protein quantitation with allthe functionality of the original DC Protein A

Strany 4 - Section 5 Storage

2Section 4 Assay InstructionsStandard Assay Protocol (5 ml)1Add 20 µl of DC Reagent S to each 1 ml of DC Reagent A that will be neededfor the run. Thi

Strany 5 - 3 What can I do to minimize

3 Pipet 25 µl of standards and samples into clean, dry microfuge tubes.4 Add 125 µl RC Reagent I into each tube, vortex. Incubate the tubes for 1minut

Strany 6 - Section 8 Order Information

4Note: Also see DCProtein Assay Instruction Manual for additionaltroubleshooting recommendations.Questions1 May I use a wavelength other than750 nm?2

Strany 7 - Related Materials

Section 7 References1 Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., “ProteinMeasurement with the Folin Phenol Reagent,” Journal of Bio

Strany 8

Related MaterialsCatalog # Product Description 500-0001 Bio-Rad Protein Assay Kit I, includes Dye Reagent Concentrate(450 ml) and Bovine Gamma Globu

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Strany 10 - 510-741-1000 4110107 Rev A

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