Bio-rad BioLogic Sample Loading Loops Uživatelský manuál stáhnout pdf (Strana 5)

Section 1

Introduction

1.1 DynaLoop Sample Loop Description

The DynaLoop sample loop is a large-volume sample injection loop for use with the

BioLogic DuoFlow

™

system. Available in 25 ml and 90 ml sample capacities, the DynaLoop

is used with the automated AVR7-3 injection valve.

1.2 Operation

The DynaLoop consists of five major components (Figure 1):

• Sample end assembly

• Buffer end assembly

• Glass tube graduated in 1 ml increments

• Sliding seal assembly

• Protective plastic jacket

Fig. 1. DynaLoop 25 and DynaLoop 90 sample loops.

The sliding seal assembly divides the glass tube into two distinct chambers:

• Sample chamber

This end connects to port 3 on the AVR7-3 injection valve.

• Buffer chamber

This end connects to port 6 on the AVR7-3 injection valve.

The DynaLoop sample loop delivers sample to the column in a four-phase process:

• Load phase

• Injection phase

• Flush phase

• Reload phase

Sample end

assembly

Sliding seal

assembly

Glass tube

Buffer end

assembly

Plastic jacket

10 1

5 20

0 10 15 20 25 30 35 40 45 50 55 60 657075 80 85 90

1 2 3 4 5 6 7 8 9 10 ... 19 20

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Bio-rad BioLogic Sample Loading Loops Uživatelský manuál Strana 5