Bio-rad Biofuel Enzyme Kit Uživatelský manuál stáhnout pdf (Strana 9)

Please read and understand steps 7–9

fully before proceeding. These steps are

time sensitive!

7. Using a clean DPTP, pipet 750 µl of

enzyme into your 15 ml conical tube of

substrate labeled “High Concentration

Substrate”.

8. Using a clean DPTP, pipet 750 µl of

enzyme into your 15 ml conical tube of

substrate labeled “Low Concentration

Substrate”. START YOUR TIMER.

9. At the times indicated, use the correctly

labeled DPTP to remove 500 µl from the

15 ml centrifuge reaction tubes labeled

“High Concentration Substrate” and “Low

Concentration Substrate” and add it to

the appropriately labeled cuvette that

contains the stop solution.

10. Proceed with the analysis of your

samples. After you have finished your

analysis, rinse out the reaction tubes,

cuvettes, and DPTPs with copious water

and save them for later activities.

Note: Do not discard unused stock solutions.

They will be used for the next activity.

34

QUICK GUIDE

Quick Guide

Enzyme

High concentration substrate

Enzyme

Low concentration substrate

High

concentration

substrate reaction

H1

1 min

H2

2 min

H3

8 min

Low

concentration

substrate reaction

L1

1 min

L2

2 min

L3

8 min

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