Bio-rad Comparative Proteomics Kit II: Western Blot Module Uživatelský manuál stáhnout pdf

Quick Guide

QUICK GUIDE

1ml

Comparative Proteomics Kit II:

Western Blot Module – Quick

Guide

Lesson 1 Quick Guide

1 Label one 1.5 ml fliptop micro tube for each of

five fish samples. Also label one screw-cap micro

tube for each fish sample.

2. Add 250 µl of Bio-Rad Laemmli sample buffer to

each labeled fliptop microtube.

3. Cut a piece of each fish muscle about 0.25 x 0.25

x 0.25 cm

( ) and transfer each piece into a

labeled fliptop micro test tube. Close the lids.

4. Flick the microtubes 15 times to agitate the tissue

in the sample buffer.

5. Incubate for 5 minutes at room temperature.

6. Carefully transfer the buffer by pouring from each

fliptop microtube into a labeled screw-cap

microtube. Do not transfer the fish!

7. Heat the fish samples in screw-cap microtubes

for 5 minutes at 95°C.

Lesson 2 Quick Guide

1. Set up Mini-PROTEAN 3 gel box.

2. Prepare a Ready Gel cassette by cutting

along the black line on the bottom of the

cassette with a razor blade and pulling off

the plastic strip, as indicated on gel

cassette.

3. Remove the comb from the Ready Gel

cassette.

4. Place Ready Gel cassette into the

electrode assembly with the short plate

facing inward. Place a buffer dam or

another Ready Gel cassette on the

opposite side of the electrode assembly,

with notch on buffer dam facing inward.

1 2 3 4 5 6 7 8 9

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Strany 1 - Western Blot Module – Quick

25Quick GuideQUICK GUIDE1mlComparative Proteomics Kit II:Western Blot Module – QuickGuideLesson 1 Quick Guide1 Label one 1.5 ml fliptop micro tube for

Strany 2 - QUICK GUIDE

5. Slide gel cassette, buffer dam, andelectrode assembly into the clampingframe.6. Press down the electrode assembly whileclosing the two cam levers o

Strany 3

27Quick GuideQUICK GUIDE11. Heat fish samples and actin and myosinstandard to 95°C for 2–5 min.12. Load your gel:Lane Volume Sample1 & 2 empty Emp

Strany 4

28QUICK GUIDEQuick GuideBanana PlugsAnode Banana Plug(Red)Notch onU-Shaped GasketGel SandwichPressure PlateCamsMini TankInnerChamberAssemblyClampingFr

Strany 5 - Lesson 3 Quick Guide

Lesson 3 Quick Guide1. Using a ruler, chop the top and bottom offthe gel. 2. Equilibrate the gel in blotting buffer for 15 minutes on a rocking pla

Strany 6

QUICK GUIDEQuick Guide6. Set up the Mini Trans-Blot module with theblack side of the cassette next to the blackside of the Mini Trans-Blot module. Ad

Strany 7

Fig. 5. Assembly of the Mini Trans-Blot cell.31QUICK GUIDEQuick GuideElectrodemoduleLidFiber padBlotting paperMembraneGelBlotting paperFiber padGel h

Strany 8 - Lesson 4 Quick Guide

Lesson 4 Quick Guide1. If not blocked overnight, immerse membrane in 25 ml blocking solution for 15 minutes to 2 hours at room tempera-ture on a rock

Strany 9 - Laboratories, Inc

Life ScienceGroup06-0372 0606 Sig 1205Bulletin 5478 US/EG Rev ABio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 4BIORAD Austra

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Bio-rad Comparative Proteomics Kit II: Western Blot Module Uživatelský manuál

Procházejte online nebo si stáhněte Uživatelský manuál pro Měřící nástroje Bio-rad Comparative Proteomics Kit II: Western Blot Module. Bio-Rad Comparative Proteomics Kit II: Western Blot Module User Manual Uživatelská příručka

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